WebTHP-1 cells show a large, round single-cell morphology and express distinct monocytic markers. Nearly all THP-1 cells start to adhere to culture plates and differentiate into macrophages after exposure to phorbol-12-myristate-13-acetate (PMA, also known as TPA,12-O-tetradecanoylphorbol-13-acetate; see below for details). WebSigma-Aldrich). THP1 cells (510 5/ml) were differentiated with 20 ng/ml PMA (Sigma-Aldrich) for 2 days to obtain THP1-MACs. Blood was obtained from healthy volunteers, according to a protocol approved by the Ethics Review Committee of The Ottawa General Hospital. Donors gave written, informed consent. Human PBMCs were isolated by Ficoll …
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WebMay 1, 2024 · However, the reduced proliferation rate of differentiated THP-1 cells after PMA stimulation for 24 h did not influenced by either l-NAME or BAPTA-AM (Fig. 6 B; n = 5 in … WebTreatment with low‑dose PMA with RANKL and MCSF failed to induce THP‑1 cell differentiation into osteoclasts. PMA alone, or a combination of RANKL and MCSF alone, is insufficient to stimulate THP‑1 cell differentiation into osteoclasts. In the present study, a reliable human osteoclast model was established using the THP‑1 cell line. grefe tree farm
Standardized protocols for differentiation of THP-1 cells to ...
WebTHP-1 cells were treated with 10 μg/mL of PMA and 3.4% β-mercaptoethanol for 72 h prior to differentiation to macrophages . After differentiation, the RPMI-1640 was used with with 4.5 g ... THP-1 cells treated with PMA were radiolabeled with [1,2-3 H] cholesterol (PerkinElmer, Waltham, MA, USA). The cellular cholesterol efflux was ... WebDec 8, 2012 · Dose-dependent and kinetic induction of IFNA and IL12B expression in DEN-2-infected THP-1 cells with or without PMA differentiation. A series of M.O.I. of DEN-2 were assessed at post-infection 24 h, RNA samples from THP-1 (A) or PMA-differentiated THP-1 (C) cells were subjected to testing IFNA and IL12B mRNAs by SYBR Green real-time RT … WebJan 13, 2010 · Differentiated macrophages are the resident tissue phagocytes and sentinel cells of the innate immune response. The phenotype of mature tissue macrophages represents the composite of environmental and differentiation-dependent imprinting. Phorbol-12-myristate-13-acetate (PMA) and 1,25-dihydroxyvitamin D3 (VD3) are stimuli … greffage imports