WebOct 12, 2024 · Cells were then pelleted by centrifugation and RNA was isolated using an RNeasy Mini Kit (Qiagen; 74104). cDNA synthesis was done using a ReadyScript cDNA Synthesis Mix (Millipore Sigma; RDRT). WebMay 8, 2015 · The cDNA was amplified using GoTaq green master Mix (Promega) using 1 µM of forward and reverse primers specific to each gene and 3 µL (60 ng) of cDNA template in a total volume of 20 µL. The thermal cycling conditions are 95 °C for 3 min, followed by 40 or 30 cycles of 95 °C for 10 s then 62 °C for 45 s for cDNA samples synthesized from …
Cdna Synthesis at Thomas Scientific
WebGeneral description. ReadyScript ® cDNA Synthesis Mix is a sensitive and easy-to-use solution for two-step RT-PCR. This 5X concentrated mix provides all necessary … WebSigma-Aldrich: RDRT: ReadyScript® cDNA Synthesis Mix Complete reagent for first strand cDNA synthesis for RT-qPCR: 100Reactions: $824: 2024-01-07: Buy: Sigma-Aldrich: ... ReadyScript cDNA Synthesis Mix is a sensitive and easy-to … shannon putman
Reverse Transcription Protocol - Sigma-Aldrich
WebJun 20, 2024 · The cDNA was synthesized with the ReadyScript TM cDNA synthesis mix (Cat. no. RDRT, Sigma Aldrich, Darmstadt, Germany) using 1µg of total RNA. RNA extraction and cDNA synthesis were executed according to the reagent manufacturer protocols. 2.6.2. WebThe SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a 2-tube format with the VILO Reaction Mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced ... WebThe SuperScript™ III First-Strand Synthesis SuperMix is an optimized SuperMix formulation for first-strand cDNA synthesis from purified poly (A) + or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 0.1 pg to 5 µg of total RNA. Using the SuperScript™ III First ... shannon purvis barron tattoos