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WebbPrinciple of the Assay HIS ELISA kit uses an anti-HIS antibody and an HIS-HRP conjugate in a competitive enzyme immunoassay method. HIS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. WebbEnzyme-linked immunosorbent assay (ELISA) was used according to the reagent instructions to detect PSEP and HSP70 in the urine samples of 210 cases of chronic … Webbstitute (Nanjing, China). e enzyme-linked immunosor-bent assay (ELISA) kits TNF-α (ml002095), IL-1β (ml063132), and IL-6 (ml063159) were purchased from Shanghai … burning godzilla fire rodan