Arabidopsis thaliana seeds were stratified at 4 °C for 2 days and sown in soil. Plants were grown in a controlled environmental chamber under the following conditions: 23 °C, 70% relative humidity, a 10-h light/14-h dark photoperiod, and a light intensity of 85 µE/m2/s. N. benthamiana plants were grown in the growth … See more A. thaliana ecotype Columbia (Col-0) was used as the WT. Arabidopsis T-DNA insertion lines in the Col-0 background for pire-1 (SALK_079510) and pire-2 … See more For co-immunoprecipitation in N. benthamiana, pENTR-LTI6b-3xFLAG and pENTR-RBOHD were recombined into pEarlygate104 to generate YFP-tagged fusions. … See more The N-terminus (aa1–376) and the C-terminus (aa756–912) of RBOHD cDNA was PCR amplified and directionally cloned into pENTR/D-TOPO (Invitrogen). The … See more In vitro kinase assays were performed as described in previously25. 0.5 μg of purified HIS-PBL13, HIS-BIK1, and HIS-PBL13ΔC and 2 μg of HIS-RBOHD-C were … See more WebMAPK phosphorylation), disease resistance, and HR [21,22]. Intriguingly, RBOHD, a NADPH oxidase that mediates ROS production, is coregulated by the two pathways, in that NLR …
Intimate Association of PRR- and NLR-Mediated Signaling in Plant ...
WebRBOHD is mainly controlled by Ca(2+) via direct binding to EF-hand motifs and phosphorylation by Ca(2+)-dependent protein kinases. Recent studies have, however, revealed a critical role for a Ca(2+)-independent regulation of RBOHD. WebFurther, we show that enhanced, as well as basal, ROS production triggered by AtPeps is absent in the double mutant of the respiratory burst oxidase homologs D and F (rbohD … imi waschpulver
Molecular Cell Article
WebThe plasma-membrane-associated kinase BIK1, which is a direct substrate of the PRR complex, directly interacts with and phosphorylates RBOHD upon PAMP perception. BIK1 … WebPhylogenetic analysis suggests that phospho-sites in the C-terminal region of RBOHD are conserved throughout the plant lineage and between animals and plants. We propose that … WebDec 2, 2024 · Previous research has shown that phosphorylation of RbohD at the C or N terminal was necessary for plant resistance to pathogens and conserved across plant … imi watson smith ltd