Web11 nov. 2024 · Seahorse Mito Stress Test. The Seahorse XFe96 Analyzer (Agilent) was used to assess metabolic activity of differentiated cardiomyocytes according to the manufacturer’s instructions (additional details available in the Methods section of the online-only Data Supplement). The XF Cell Mito Stress Test Kit was used according to the … WebThey have done seahorse comparing two cell lines (WT and KD for gene X) as part of their study, specifically the mito-stress protocol (oligomycin; FCCP; Rot/AA). They have nice OCR data and can draw several observations from that, but they were also wondering how they can exploit the ECAR data from the mito-stress conditions.
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WebThe Seahorse XF Cell Mito Stress Test can help quantify multiple parameters of mitochondrial respiration and identify mitochondrial dysfunction. See what … WebFigure 2︓悪性⿊⾊腫エクソソームmiRNAによって誘発される代謝の変化。A、B) Seahorse XF Glycolytic Rate Assayは悪性⿊⾊腫エク ソソームmiRNAによって駆動される解糖の増加を明らかにする。C、D) Seahorse XF Cell Mito Stress Testは悪性⿊⾊腫エク … do corn tortillas raise blood sugar
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WebFunctional mitochondrial toxicity assessment protocol. 50 µL of 50 mM DMSO solution or equivalent amount of solid compound. Use of solid state fluorescent sensors to measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). Measured using the XF e 96 flux analyzer (Seahorse Biosciences Inc) Web30 dec. 2024 · The Cell Mito Stress Test is a common method to measure the key parameters of mitochondrial respiration. Here, we use the human cell line HK-2 as an … Web25 mrt. 2024 · Analyzing mitochondrial stress test and fatty acid oxidation in dendritic cells Graphical Abstract Before you begin Immunometabolism is an emerging field of investigation at the interface between the historically distinct disciplines of immunology and metabolism ( Mathis and Shoelson, 2011 ). do corporate credit cards check your credit