Irf3 phosphorylation antibody
WebOct 22, 2024 · Phosphorylation occurs when IRF3 is recruited to adaptor proteins via a positively charged surface within the regulatory domain of IRF3. This positively charged surface also plays a crucial role in forming the active homodimer by interacting with the phosphorylated sites stabilizing the homodimer. WebSep 1, 2024 · Immunoblot analysis of IRF3 with anti-phospho-Ser82 antibody and IRF3 antibody in HEK293 cells untreated or infected with SeV (50 HA units/ml) for 8 hr, ... Phosphorylation on IRF3 could turn on or off its function, corresponding to positive or negative regulation, respectively. The positive and negative regulation phosphorylation, …
Irf3 phosphorylation antibody
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WebMar 5, 2024 · Rnf39 deficiency and knockdown both enhanced RNA virus–induced IRF3 phosphorylation (Fig. 2, A to C, and fig. S3D). Furthermore, ... The cell lysates were immunoprecipitated with Flag antibody and then immunoblotted with the indicated antibodies. The specific bands were marked by asterisk. All experiments were repeated at … WebViral infection results in phosphorylation of IRF3, leading to its translocation to the nucleus, where it activates promoters containing IRF3-binding …
Web(C,D) 4b(Δ2-38) inhibits the phosphorylation and nuclear translocation of IRF3. Experiments were carried out as in AB, except that 4b(Δ2-38) was accessed. Source publication WebInvitrogen Anti-Phospho-IRF3 (Ser396) Polyclonal, Catalog # 720012. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. This antibody reacts with Human …
WebQing Yang, Hong-Bing Shu, in Advances in Immunology, 2024. 2.4.5 Regulation of IRF3 activity. Upon viral infection, IRF3 undergoes phosphorylation at S396 by TBK1, and … WebUse Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3. Anti-phospho …
WebCustom Monoclonal Antibodies; CellPower™ Assay Cell Line Development; DIAGNOSTICS Hot! COVID-19. cPass™ Technology . Rapid Tests. ... Tyrosine phosphorylation of the E3 ubiquitin ligase TRIM21 positively regulates interaction with IRF3 and hence TRIM21 activity. PLoS One.. 2012-03; 7(3): ...
WebAll mutants lacked phosphorylation of the STING-S365 residue, IRF3, and STAT1 upon stimulation, indicating defects in IRF3 activation and type I IFN signaling (Fig. 1A and SI … dhaval acharyaWebKey features and details Rabbit polyclonal to IRF3 (phospho S396) Suitable for: WB Reacts with: Human Isotype: IgG You may also be interested in Primary Anti-IRF3 (phospho S386) … cif one motorsWebPhospho-IRF3 (Ser396) Antibody (MA5-14947) in WB Frontiers in microbiology 2024 - FIGURE 2 Effects of Set-Met on the expression of MAVS protein and the phosphorylation … dh autos rallyeWebPhospho-IRF3 (S386) ELISA Kit (ab279833) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated IRF3 protein in your experimental model system, you can verify pathway activation in your cell lysates. cif on chequeWebAnti-IRF3 antibody [IRF35I218] (ab50772) Datasheet Reviews ( 3) Q&A (4) References (9) Key features and details Mouse monoclonal [IRF35I218] to IRF3 Suitable for: ICC/IF, WB, Flow Cyt Reacts with: Human Isotype: IgG1 You may also be interested in Primary Alexa Fluor® 488 Anti-PSMA antibody [GCP05] (ab187570) cif one.comWebAn unbiased structure-guided alignment of full-length IRF3, MAVS, and STING across species revealed sequence similarity among the MAVS 4T/S cluster, STING C terminus … dhaval and guroudev productions pvt. ltdWebAll mutants lacked phosphorylation of the STING-S365 residue, IRF3, and STAT1 upon stimulation, indicating defects in IRF3 activation and type I IFN signaling (Fig. 1A and SI Appendix, Fig. S1A). Consistently, all mutants were deficient in IRF3 nuclear translocation ( Fig. 1 B and SI Appendix , Fig. S1 B ) and did not produce IFNβ after DMXAA ... cif onet seralia