WebSpecifically, when TTC is applied to slices of brain or heart tissues following induction of infarct (e.g. middle cerebral artery occlusion (MCAo), the viable tissue is stained red, … Web(A) TTC staining. (B) The heart gross morphological image. (C) H&E staining of Crosssections of the hearts at the papillary muscle level and the infarct section (scale …
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Web9 de abr. de 2024 · For permanent occlusion MI models, infarct size must be measured in fresh LV slices at the time of necropsy by TTC staining and typically ranges from 30% to 60% of the total LV (47, 48, 61, 89, 98, 152, 195, 202, 206, 223, 310–312, 319, 323). The method for calculating infarct size varies across laboratories. Web23 de may. de 2024 · TTC staining allows identification of MI as area colored off-white (example indicated by arrow). Evans blue staining allows identification of AAR as area not stained blue (example indicated... gray fox size
Using ImageJ to measure areas in histological samples - YouTube
Web4 de abr. de 2024 · Finally, TTC staining was performed to evaluate the infarct size at 24 h after I/R injury, and heart tissue harvest was performed at 1 week post I/R injury. In addition, 8- to 10-week-old male miR-486 KO mice and WT littermates received swimming exercise for 3 weeks and then were subjected to cardiac I/R injury for determination of the infarct … Web19 de ago. de 2013 · Deminstration using ImageJ software to measure areas of colour on histological samples. In thgis examle I am using a 750µm slice of an infarcted heart staine... Web13 de jul. de 2015 · The heart was then excised and perfused with saline and cut into sequential 2-mm-thick cross sections. The sections were incubated with 1% 2,3,5-triphenyltetrazolium chloride (TTC, Sigma Aldrich) for 10 minutes at 37°C and were photographed with a stereomicroscope (Nikon, HC-2500). chocolatier boon