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Cst myc antibody

WebApr 13, 2024 · DNA fragments were incubated overnight with specific antibody c-Myc (E5Q6W, CST, USA) and control IgG. The next day, antibody-binding magnetic beads were added. Proteinase K was used to elute and digest the immunoprecipitated product. The purified DNA product was used for subsequent PCR detection (Table S2). Mouse IgG … WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-c-Myc (phospho S62) antibody [EPR17924] (ab185656) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – …

Anti-HA tag antibody [HA.C5] (ab18181) Abcam

Web6x-His Tag Antibody (MA1-21315) in ICC/IF. Immunofluorescent analysis of 6x-His Tag was performed using 70% confluent HEK-293 cells transfected with V5-H3-His construct. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature ... WebCST complex. The CST complex is a cellular multiprotein complex involved in telomere maintenance. In budding yeast ( Saccharomyces cerevisiae ), it is composed of the … iphone calendar not deleting events https://andygilmorephotos.com

Human c-Myc PE-conjugated Antibody IC3696P: R&D Systems

WebMyc-Tag (9B11) Mouse mAb detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of … WebWe hypothesized that Euonymus sachalinensis (ES) induces apoptosis by inhibiting the expression of c-Myc in colon cancer cells, and this study proved that the methanol extract of ES has anticancer effects in colon cancer cells. ES belongs to the Celastraceae family and is well known for its medicinal properties. Extracts of species belonging to this family … WebThis antibody detects c-myc protein and c-myc tagged proteins. MA1-980 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA, flow cytometry, and … iphone calendar does not sync with outlook

Myc Antibody (9E10) SCBT - Santa Cruz Biotechnology

Category:Anti-c-Myc antibody [8] (ab17355) Abcam

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Cst myc antibody

c-Myc Antibody from Cell Signaling Technology - Biocompare

WebImmunofluorescent analysis of Transfected HEK-293 cells using 66004-1-Ig (MYC-tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This product is out of stock now, and we suggest you go to the replacement 60003-2-Ig. Our concentration is detected with BSA as standard. WebHuman c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max. It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains.

Cst myc antibody

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WebMonoclonal Antibody for studying Myc. Cited in 820 publications. Validated for WB, WB, IF. Available in 2 sizes. Highly specific and rigorously … WebC – Pulmonary EC co-transfected with GFP-tagged CLIP-170, CLASP2, EB1 or β-tubulin and Myc-SOCS1 were tested in co-IP assay with GFP antibody followed by immunoblotting with Myc-antibody. Probing of total cell lysates with GFP and Myc antibodies was used to verify ectopic protein expression; membrane re-probing for β-actin was used to ...

WebThe c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA … WebMouse monoclonal c-Myc antibody [8]. Validated in IHC and tested in Human. Cited in 6 publication(s). Independently reviewed in 6 review(s). Immunogen corresponding to recombinant full length protein.

WebAnti-Myc Tag, clone 4A6, is a mouse monoclonal antibody that is validated for use in ChIP, IC, IF, IP and WB for the detection of Myc Tag and does not show the very high context sensitivity of 9E10. This antibody has been cited in over 480 publications. Research Category. Epitope Tags & General Use. Research Sub Category. WebIP. ChIP. Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and either MTA3 (E3X2E) Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP ® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR, using human Myc exon 2 primers and SimpleChIP ® …

Web13 hours ago · Our previous studies demonstrated that rapamycin and rapalogs significantly induced IRES-mediated translation of cyclin D1 and c-myc mRNAs following exposure [16, 20, 21].The ability of these compounds to induce IRES activity of these two determinants was sufficient to render cells resistant to rapamycin in an AKT-dependent manner …

WebGeneral description. c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. It belongs to the family of transcription factors which are characterized by basic region helix-loop-helix/leucine zipper. [ 1] iphone calendar invite cannot be sentWebICC staining of purified anti-c-Myc antibody (clone 9E10) on HeLa cells transfected with multi-tag plasmid containing c-Myc epitope sequence. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100, and blocked with 5% fetal bovine serum in PBS for 1 hour. The cells were then incubated with 1 µg/mL of the ... iphone calendar wrong time zoneWebThe FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions. The FLAG tag may be inserted at the N terminus, the N terminus preceded by a methionine residue, the C terminus, or internal positions of the target protein. iphone call blockerWebThe c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous ... orange bedroom ideas for boysWebMouse monoclonal c-Myc antibody [8]. Validated in IHC and tested in Human. Cited in 6 publication(s). Independently reviewed in 6 review(s). … iphone calendar what is cdtWebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. iphone call automatically going to speakerWebThe mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc. c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. iphone calendar show all events for the month